Effects of dietary restriction on the splenic B cell subtypes.

<p><b>A)</b> In the first panel, FACS plots of splenic cells plotted against CD19 and B220 in the dietary interventions groups, while the second panel shows the IgM/IgD profiles of gated CD19⁺B220⁺ fractions. The third panel represents the CD21/CD23 profiles of the CD19⁺B220⁺ fractions. <b>B)</b> Shows the total CD19⁺B220⁺ B cell cellularity in spleen. <b>C)</b> Shows that IgM^lowIgD^low was significantly affected by FA but not by DR. A similar trend was also observed with respect to cellularity of immature IgM⁺IgD⁻ and mature IgM⁺IgD⁺ populations. <b>D)</b> Represents the cellularity changes in CD21⁺CD23⁻ marginal zone and CD21⁻CD23⁺ follicular B cells. Fig. 3B ** = p<0.001, # = p<0.05. Fig. 3C # = p<0.05 **, ## = p<0.001. Fig. 3D ** = p<0.002, ## = p<0.005. n = 8/group. Ad libitum, 2 weeks 30% DR and 3 days fasting groups are represented by black, white and grey box, respectively.