Effects of dietary restriction on B cell development phenotype in the BM.

<p><b>A)</b> The first panel of FACS plots represents the plots of CD19 and B220 from the three dietary regimen groups. The second panel shows IgD and IgM populations from CD19⁺B220⁺ B cell fraction with IgM⁻IgD⁻ populations (pro-B+pre-B cells), IgM⁺IgD^low (immature B cells) and IgM^+/lowIgD^high (recirculating mature) B cells. The third panel represents the CD43⁻CD2 profiles of IgD⁻ IgM^- fractions and distinguishes between pro-B (CD43⁺CD2⁻IgM⁻IgD⁻) and pre-B (CD43⁻CD2⁺IgM⁻IgD⁻). <b>B)</b> Represents the total B-lineage population (CD19⁺B220⁺) in BM. <b>C, D)</b> shows the changes in the B cell development phenotype where both DR and FA cause significant phenotypic alternations in the development stages. Fig. 2B and C *** = p<0.0002, ### = p<0.0004. Fig. 2D *, # = p<0.05, **, ## = p<0.004, *** = p<0.0003. n = 8/group. Ad libitum, 2 weeks 30% DR and 3 days fasting groups are represented by black, white and grey box, respectively.