Gene expression profiles in subperitoneal fibroblasts (SPFs) and submucosal fibroblasts (SMFs) with and without cancer-cell-conditioned medium (CCCM) stimulation.

<p>(A) Red is the microarray profile in SMFs with CCCM stimulation, blue is SMFs without CCCM stimulation, green is SPFs with CCCM stimulation, and silver is SPFs without CCCM stimulation. Three-dimensional representation of principal component analysis (PCA) component 1, 2, and 3. (B) Two dimensional representation of PCA components 1 and 2 (upper), and PCA components 1 and 3 (lower). Fibroblasts formed independent clusters, depending on histoanatomical site and the presence of CCCM stimulation. (C) Supervised cluster analysis in fibroblasts also revealed distinct clusters depending on histoanatomical site and the presence of CCCM stimulation. (D) Gene ontology analysis of upregulated genes in SPFs compared with SMFs. (E) Gene ontology analysis of genes upregulated in SPFs with CCCM stimulation, compared with SMFs with CCCM stimulation. Most of the genes with increased expressions in SPFs were retained after CCCM stimulation; however, there were some differences, and the order of annotation clusters were changed after CCCM stimulation.