Phenotypic analysis of LIM protein mutants in <i>M. oryzae</i>.

<p><b>a</b>. The diameter of colonies grown on CM plates at 25°C for 10 d. <b>b</b>. The conidia washed from the 15d-old CM cultures. <b>c</b>. Percentage of appressorium formation on GB (GelBond) surfaces incubated for 24 h or OE (onion epidermis) surfaces incubated for 48 h at 25°C. <b>d</b>. Percentage of invasive hyphae formation from appressoria incubated on OE for 24 h at 25°C. More than 300 spores or appressoria were counted for each strain. <b>e</b>. Different letters after mean values indicated significant difference at P-value of 0.05. Data were calculated from three independent experiments conducted in triplicates. Strains: Guy11, LG25 (<i>Δrga1</i>), GC22 (<i>Δrga:RGA1</i>), LD17 (<i>Δldp1</i>), Ku80, LP55 (<i>Δpax1</i>), PC20 (<i>Δpax1:PAX1</i>), Ku70, LR80 (<i>Δlrg1</i>) and RC38 (<i>Δlrg1:LRG1</i>).