Competitive inhibition of binding to HA among the Abs newly induced by vaccination.

<p>The binding activity of Fab-PP Ab (indicated at upper side) to Cal09 virus particles was measured by ELISA under the presence of a 10-times greater concentration of Fab-cp3 Ab (indicated at the left side). F008-009 and F033-367 are not anti-HA Abs and were used as controls. The binding inhibition was calculated as follows: the absorbance value under the presence of F008-009cp3 was used as 100% binding, and the degree of reduction in the absorbance value under the presence of Fab-cp3 of Ab was measured and shown as percent inhibition. Percent inhibition is shown as follows: ≥70% (white), 50%–70% (blue), 0%–50% (grey). The experiment was performed at least three times in duplicate.