CD47 antibody attenuated TSP-1′s inhibition on angiogenesis of late EPCs.

<p>(A) Late EPCs were plated on Matrigel and treated with anti-CD47 antibody (2.5 µg/ml), anti-integrin β1 antibody (2 µg/ml) or control IgG for 30 min and then treated with TSP-1(2 µg/ml) as described (Scale bar = 400 µm, 50×magnification). (B) Total tube length per HPF (100×magnification) was measured. Values are presented as the mean±S.D. of three independent experiments (#p<0.05 versus control siRNA with TSP-1 stimulation)