Screening a novel chemical library.

<p>A) Alterations in cell number A) after 24 (open squares) or 48 hours exposure (closed triangles) exposure to 329 diverse compounds, based on Z-scores calculated from negative controls, compounds above and below the cut-offs of +2 or −2 (dotted lines) are considered hits. B) Chemical structures of compounds 137,110 and 147 which were hit compounds in the three different endpoints assessed. C) Alterations in the percentage of morphologically abnormal cells after 24 (open squares) or 48 hours exposure (closed triangles) dotted lines as for A). D) Representative images from negative control (0.2% DMSO) and hit compounds in the three different endpoints assessed; compound 137 altered cell number and compound 110 increased the percentage of morphologically abnormal cells. (DMSO, compounds 137 and 110 after 48 hours exposure) (Scale bars are 50 µm). The most active compound, 137, showed dose-responses for both cell loss E) and increased the percentage of morphologically abnormal cells F).