Effect of stimulus intensity on number of post-stimulus spikes.

<p>A) Loose cell (left panels) and whole cell (right panels) recordings from representative Purkinje neurons during stimulation in the molecular layer (arrow) at increasing intensities (as indicated). B) Loose cell recordings from representative cells during stimulation in the granular layer, at the same intensities as shown for molecular layer. C) Raster plot of number of post-stimulus spikes for 10 representative responses at each stimulus intensity. Note that 0 µA corresponds to sampling from the record in the absence of stimulus but with the same frequency as during stimulation, to give a measure of the probability of one or more spikes occurring within the sampling window by chance. D) Mean ± s.e.m. number of post-stimulus spikes against stimulus intensity (n = 15 cells). **p = 0.0053, ***p = 0.0009, *p = 0.0383, for comparison of mean to preceding (lower) stimulus intensity. Multiple comparisons ANOVA with Tukey’s test for pairs (adjusted p values). E) Raster plot of 10 representative interspike intervals, for first pair of spikes, at each stimulus intensity. F) Mean ± s.e.m. of first interspike interval against stimulus intensity (n = 15 cells). *p<0.02 compared to 5 µA. Multiple comparisons ANOVA with Tukey’s test for pairs.