Co-staining of CT carbohydrate with macrophages in GRMD muscle.

<p>(A) Single and merged staining for CT2 (green) with CD11b (red, to mark macrophages) and DAPI (blue). Arrow shows co-incident staining for CT2 and CD11b. (B) Single and merged staining for CT2 (green) with Pax7 (red, to mark satellite cells) and DAPI (blue). Arrow shows lack of coincident staining for CT2 and Pax7. (C) Examples of WFA staining (green) with embryonic myosin (red) and DAPI (blue) (two left panels). Examples of control staining for staining shown in A and B using only secondary antibodies with DAPI (two right panels). Bar is 50 µm for all panels in A, B and C.