Expression of giant obscurins in rodent tissues and organs.

<p>Western blot analysis of 70 µg of protein homogenates prepared from adult mouse (A) and rat (B) tissues were probed with four antibodies to obscurins: α-NH₂, α-COOH, α-ABD, and α-Kinase (the epitope for each antibody is noted in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088162#pone-0088162-g001" target="_blank">Figure 1</a>). The blots have been cut at ∼600 kDa to focus on the giant forms of obscurin, and representative lanes from multiple experiments are shown. In agreement with previously published data, giant obscurin-A and -B are consistently identified in both cardiac and skeletal muscles of mouse and rat origin using any of the four obscurin antibodies. Notably, they are also present in select non-muscle tissues including brain, skin, and lung. Similar protein content per lane was ensured with a GAPDH load control.