Transduced WEHI-231 B cell lines express host Bcl-2 or viral Bcl-2 (M11) proteins.

<p>Parental WEHI-231 cells (WEHI) were transduced with empty retroviral vector (WEHI.EV) or with retroviruses expressing host Bcl-2 (WEHI.Bcl-2) or MHV68 vBcl-2 (WEHI.M11.1 and WEHI.M11.2) to generate stable cell lines. Western blots were performed to confirm expression of (<b>A</b>) cellular Bcl-2 or (<b>B</b>) viral Bcl-2. Whole cell lysates were collected and subjected to immunoblotting to detect cellular Bcl-2 (26 kDa) and actin (43 kDa) or HA-tagged vBcl-2 (18 kDa) and actin (43 kDa). Data shown is representative of 3 individual experiments.