Expression profiling of a cluster of MiRNA genes within the <i>Hstx1/Hstx2</i> critical region.
- Tanmoy Bhattacharyya (585402)
- Radka Reifová (399704)
- Soňa Gregorová (124489)
- Petr Simecek (99422)
- Vaclav Gergelits (585403)
- Martin Mistrik (585404)
- Iva Martincova (585405)
- Jaroslav Piálek (124496)
- Jiri Forejt (24636)
Publication date
February 2014
DOIAbstract
<p>(A) Log fold-change of expression ratio of PWD versus B6 MiRNA genes in flow-sorted testicular cells. Significant overexpression of Mir465 cluster in PWD germ cells is shown in red. (B) Validation of Mir465 overexpression in PWD primary spermatocytes by qRT PCR. Data normalized to U6 non-coding RNA (C) Log fold-change of expression ratio of MiRNA genes in (PWD×B6)F1 versus (B6×PWD)F1 (abbreviated PB6F1 and B6PF1) 14.5 d old testes. Significant upregulation in red (P<0.05). (D) qRT PCR validation of differences in Mir465 expression. Data normalized to Mir152.</p
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