Immunolocalization of Enzyme Positive Cells before Fracture.

<p>Top image shows a mouse femur stained with safranin-O (orange) and counter stained with fast green (green) and hematoxylin (black; CB: cortical bone; scale bar: 300 um). Bottom images show immunohistochemical staining of different cell types with antibodies to COX-1, COX-2, 5-LO, or LTA4H (brown; scale bar: 50 um). Rabbit IgG was used as a negative control (Neg.). Immunohistochemistry specimens were counter stained with methyl green. The higher magnification images are labeled with the primary antibody target (COX-1, COX-2, 5-LO, LTA4H, or Neg.) and with a letter indicating cell type and location as listed in the bottom of the figure.