CD34 and CD200 were early-stage markers of hair follicle stem cells by Immunohistochemistry (100X).

<p>(A) ESCs-C-CBM shows CD34 expressed in the hair follicle bulge cells at d3 after surgery and maintained from d3 to 4 w, and then decreased after 6 w compare to group C-CBM, which shows CD34 was expressed 4 w after surgery; (B) CD200 was located at the hair follicle group ESCs-C-CMB. It was expressed at d3 after surgery and maintained until 6w compare the control group C-CMB which CD200 was expressed at d 7 and the expression decreased from 4w. (C) Measurement of early-stage <i>in vivo</i> CD34/CD200 markers of hair follicle stem cells in the epidermal nests. Hair follicle longitudinal sections and hair follicle transverse sections are both shown. (D) CD34 and CD200 present on ESCs during early stage <i>in vivo</i>. CD34 was strongly expressed in the hair bulb at d5. CD200 was highly expressed at the outer hair root sheath at d3.