Effects of atenolol, perindopril, and nifedipine on high glucose-induced cellular senescence in HUVECs.

<p><b>(A)</b> Cytochemical staining for SA-β-gal activity. Cells were incubated with 22 mM glucose for 3 days in the absence and presence of 10 µM atenolol, 10 µM perindopril, or 1 nM nifedipine. Bar = 100 µm. <b>(B)</b> A bar graph summarizes the results from 5 experiments shown in <b>A</b>. <b>(C)</b> Telomerase activity was measured by the telomere repeat application protocol (trap) assay (n = 4). ***<i>P</i><0.001 versus NG (5.5 mM glucose). ##<i>P</i><0.01, ###<i>P</i><0.001 versus HG (22 mM glucose) alone.