Expression of 2APro driven by pU2A construct leads to cleavage of eIF4G-I.

<p>A. HeLa cells were co-transfected with pU2A and the empty vector pCDNA3.1 at proportion 1∶3 or mock-transfected and incubated for 24, 48 or 72 hours. After this time, cell lysates were processed by SDS-PAGE followed by western-blotting with an anti-eIF4G-I antibody. B. HeLa cells were co-transfected with pU2A and the empty vector pCDNA3.1 at different proportions (1∶3, 1∶1 and 3∶1) and incubated for 48 hours. After this time, cell lysates were processed as in A.