Cre-recombinase-mediated tissue-specific excision of <i>Ggcx</i>.

<p>A, Strategy for homologous recombination. Targeting vector was designed to flank exon 6 of the <i>Ggcx</i> gene, and frame shift was generated by excision with Cre recombinase. Two <i>loxP</i> sequences (triangles) were inserted into introns 5 and 6. Neomycin cassette and EcoRI site were inserted into intron 6. B, Southern blot analysis of tail DNA. Homologous recombinant allele generated a 7.4-kb fragment by EcoRI digestion. A representative figure is shown.