CHOP dependent induction of endothelial nitric oxide synthase (eNOS) in BC treated cardiomyocytes.

<p>(A) Cardiomyocytes were treated with Tm (100 ng/ml) in the presence or absence of baicalin (BC) for 24 h at indicated concentrations (12.5, 25, 50 µM). The expressions of eNOS and phospho eNOS at protein levels were examined by western blot. (B) BC treatment increased NO production in cultured cardiomyocytes. Cardiomyocytes were treated with Tm (100 ng/ml) in the presence or absence of baicalin (BC) for 24 h and incubated with 10 µM of DAF-FM and fluorescence intensity was assayed. (C) CHOP knockdown efficiency was tested by western blot. Cells transfected with either control siRNA or siRNA targeting CHOP were further treated with Tm (100 ng/ml) in the presence or absence of baicalin (BC, 25 µM) for 24 h. (D) The expression of eNOS mRNA levels was examined by real-time quantitative PCR. Cells were treated as C. (E) The expressions of eNOS and phospho eNOS protein levels were examined by western blot. Cells were treated as C. (F) NO production was measured by DAF-FM and fluorescence intensity was assayed. Cells were treated as C. A, B: *P<0.05 vs Ctrl, **P<0.05 vs Tm, n = 5; C, D, E, F: *P<0.05 vs Control+si NC, **P<0.05 vs Tm+si NC, n = 5.