Minimum spanning tree of MLVA data from study isolates (n = 47).

<p>Letter symbol in the center of the circle represents the results for testing by cytotoxin assay for 42 samples included in the analysis (C + and C −). Five samples were not tested by CYT (NT). Each circle represents a distinct MLVA type and numbers between the circles represent the STRD [Summed tandem repeat difference]. Isolates with a STRD<10 are highlighted in colored clouds representing clusters (genetically related clonal complexes). <i>tcdC</i> sequencing is depicted by color coding within circles with <i>tcdC 1</i> (corresponding to NAP1) strain represented in red (reference in right corner).