BRK and Dok1 are differentially overexpressed in the human breast cancer cell lines.

<p>(A) Cellular proteins were detected in total cell lysates by immunoblotting analysis with anti-Dok1 and anti-BRK antibodies. β-tubulin expression served as a loading control. (B & C) SKBR3 and BT20 cells were fractionated into the cytosolic, membrane, nuclear and cytoskeleton fractions and subjected to immunoblotting analysis for the detection of BRK and Dok1. β-tubulin and Sam68 were used as controls for the cytosolic/membrane and nuclear compartments, respectively. (D) Stable BRK knockdown was performed on parental breast cancer cell lines SKBR3 using shRNA lentiviral vector plasmids against BRK and analyzed as indicated.