Upregulation of Fcγ receptors expression in injured mouse nerves.

<p>Quantitative PCR showing the relative mRNA levels of FcγRI, FcγRII, FcγRIII, and FcγRIV in injured nerves at various time points after surgery (A). Single labeling immunocytochemistry studies showing the expression of Fcγ common chain (shared by all activating Fcγ receptors) in the injured sciatic nerves (B). Western blotting images and quantitative densitometry of Fcγ common chain in the injured nerves (C). Double labeling immunocytochemistry studies showing the activating Fcγ receptors by endogenous {microglia (Iba1 positive; 1 day after injury) and Schwann cells (GFAP positive; 4 days after injury} and recruited {macrophages (CD68 positive); 14 days after injury} glia in the injured nerves (D). *<i>p</i> < 0.001 (One-way ANOVA, Bonferroni’s’ post-hoc test). Arrows showing the co-localization between activating Fcγ receptors signal (Fcγ common chain) and glia cell markers (GFAP and Iba1). N = 4–5 per group. Error bars, s.e.m. Scale bar, 10 µm.