Differences in lag phase between cultures with and without the addition of spent medium supernatant.

<p>Measured by the Bioscreen instrument: example of one experiment. Dormant cells of SH1000, aged 7 days, with and without addition of 50% spent medium supernatant derived from SH1000 cultures: five replicate cultures. <i>Solid black lines</i>: increasing absorbance values of aged cultures inoculated in fresh minimal medium without supplementation. <i>Dashed lines</i>: increasing absorbance values of aged cultures inoculated in supplemented fresh minimal medium. Difference in lag phase as indicated by OD600 reaching >0.1 was measured.