Establishment of transgenic plants.

<p>A. Time course of establishment of transgenic T₀ plants from cotyledonary node explants (n = 160). Each line indicates the frequency of Agrobacterium-infected explants that reached the process indicated in (B). B. Schematic representation of the entire transformation procedure. C. Green fluorescent images of T₁ seedlings of a single T-DNA insertion line (#1 in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088611#pone-0088611-g006" target="_blank">Figure 6</a>). The zygosity of T₁ progeny [non-transformant (−/−), hemizygote (Tg/−) or homozygote (Tg/Tg)] produced by self-crossing of a T₀ plant could be determined based on fluorescence intensity of the plantlet. D. Green fluorescent image of homozygous T₂ seedling produced by self-crossing of a homozygous T₁ plant. Scale bar, 2 mm.