<p>The T7 RNA polymerase binding site is in bold in the reverse primers. F = Forward primer; RT7 = Reverse primer; MO = targeting morpholino; ATG MO = targets translation initiation; Splice MO = targets splicing event; 5MM = control morpholino with 5 mismatch pairs to target sequence. All primers and MOs are shown in the 5′-3′orientation.</p
<p>F and R indicate the forward and reverse directions, respectively. The underlined regions represe...
AbstractNew crystal structures of transcription complexes formed by bacteriophage T7 RNA polymerase ...
a<p>Nucleotides in bold indicate restriction sites introduced for cloning.</p>b<p>T7 RNA polymerase ...
<p>Lines superposed over the alignment show the major features obtained experimentally for T7 RNAP. ...
The functional significance of the conserved motif TX2GR, included in one of the six main regions of...
<p><sup>a</sup>Capital letters indicate coding regions, underline indicates restriction enzyme sites...
a<p>For each truncation construct, forward primer (F) is in the forward orientation relative to the ...
A site-specifically modified oligonucleotide containing a single 2′-deoxyribonolactone lesion was us...
<p>Primers with 454-Adaptors (A and B) used to amplify the overlapping protease- and reverse-transcr...
<p>(G) A schematic of the jump from the cavity model, depicting the key lysine/arginine module that ...
Eberwine(-like) amplification of mRNA adds distinct 6-10 bp nucleotide stretches to the 5' end of am...
<p>Multiplex identifiers (MIDs) are underlined. Forward primers use MID7 and reverse primers use MID...
Eberwine(-like) amplification of mRNA adds distinct 6-10 bp nucleotide stretches to the 5' end of am...
The use of the T7 RNA polymerase is ubiquitous in molecular biology as a research tool for the study...
efor base selectivity, and the question arises of why tran-residues of the RNA transcript hybridized...
<p>F and R indicate the forward and reverse directions, respectively. The underlined regions represe...
AbstractNew crystal structures of transcription complexes formed by bacteriophage T7 RNA polymerase ...
a<p>Nucleotides in bold indicate restriction sites introduced for cloning.</p>b<p>T7 RNA polymerase ...
<p>Lines superposed over the alignment show the major features obtained experimentally for T7 RNAP. ...
The functional significance of the conserved motif TX2GR, included in one of the six main regions of...
<p><sup>a</sup>Capital letters indicate coding regions, underline indicates restriction enzyme sites...
a<p>For each truncation construct, forward primer (F) is in the forward orientation relative to the ...
A site-specifically modified oligonucleotide containing a single 2′-deoxyribonolactone lesion was us...
<p>Primers with 454-Adaptors (A and B) used to amplify the overlapping protease- and reverse-transcr...
<p>(G) A schematic of the jump from the cavity model, depicting the key lysine/arginine module that ...
Eberwine(-like) amplification of mRNA adds distinct 6-10 bp nucleotide stretches to the 5' end of am...
<p>Multiplex identifiers (MIDs) are underlined. Forward primers use MID7 and reverse primers use MID...
Eberwine(-like) amplification of mRNA adds distinct 6-10 bp nucleotide stretches to the 5' end of am...
The use of the T7 RNA polymerase is ubiquitous in molecular biology as a research tool for the study...
efor base selectivity, and the question arises of why tran-residues of the RNA transcript hybridized...
<p>F and R indicate the forward and reverse directions, respectively. The underlined regions represe...
AbstractNew crystal structures of transcription complexes formed by bacteriophage T7 RNA polymerase ...
a<p>Nucleotides in bold indicate restriction sites introduced for cloning.</p>b<p>T7 RNA polymerase ...
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