RIM1α/CB1R ratio levels determine synaptic efficiency in HU-210 treated cells.

<p>A) Post hoc immunocytochemical images of FM1-43 loaded synaptic boutons in blue (pseudocolor), anti-CB1R in red and anti-RIM1α in green. Selected areas in A are shown at higher magnification in C. Normalized FM1-43 unloading of synaptic boutons according to: RIM1α (B) and RIM1α/CB1R ratio (D) (whole population in blue; subpopulation with protein or ratio value >2 in red; subpopulation with protein or ratio value <0.5 in black). E) examples of FM1-43 unloading kinetics of the individual synaptic boutons shown in C. Traces in B are means of n = 541 synaptic boutons from 4 covers in whole population of synaptic boutons; n = 91 in RIM1α >2; n = 219 in RIM1α<0.5. Traces in D are means of n = 541 synaptic boutons from 4 covers in whole population of synaptic boutons; n = 48 in RIM1α/CB1R >2; n = 55 in RIM1α/CB1R<0.5. Scale bar 25 µm. ^NSp>0.05, **p<0.01 compared to whole population (Kolmogorov-Smirnov test).