Expression of phospho STAT3 in scrambled control and RhoC knockdown HNSCC cell lines.

<p>(A and B) Western blot analysis showing the phosphorylation of STAT3^ser727 and STAT3 ^tyr705 in the scrambled controls and the RhoC knockdown UM-SCC-1 and -47 respectively. Normalized value with respect to total STAT3 is given as the numerical values. Remarkable decreases in the phosphorylation of STAT3 were seen in the RhoC knock-down UM-SCC-1 and -47 cell lines respectively. (C) Protein analysis shows the p-STAT3^tyr705 in the ectopically overexpressed (OE) scrambled control and the RhoC knockdown UM-SCC-1. Phosphorylated form was detected only in the scrambled control when STAT3 was over expressed. A thick band of total STAT3 can be seen in the lower panel, confirming the successful transfection of STAT3 in these clones. (D) Real time RT-PCR showing the expression of STAT3, sox2, oct3/4 and nanog before and after the STAT3 over expression in the RhoC knockdown clone. The mRNA expression of STAT3 was dramatically high after it's over expression, while no significant change in the expression was observed in sox2, oct3/4 and nanog in the RhoC knockdown clone.