ALDH expression and tumorsphere formation in scrambled control and RhoC knockdown UM-SCC- cell lines.

<p>FACS analysis showing the expression of (A) ALDH in the scrambled control and the RhoC knockdown clones. (B) The tumorspheres formation in the scrambled control and the RhoC knockdown UM-SCC-1 and -47cell lines. (C) The representation of tumorspheres formation efficiency in scrambled control and RhoC knockdown UM-SCC-1 obtained in 96 well plates. A significant decrease in ALDH expression was seen. Tumorsphere formation was dramatically low in RhoC knockdown UM-SCC-1 and -47 respectively. (D) A significant decrease in the RhoC mRNA expression was obtained in the RhoC knockdown adherent cell and in the tumorspheres. (E) Stem cell transcription factors, sox2, oct3/4, and nanog showing significant down regulation in their mRNA as revealed by real time RT-PCR in the scrambled control and the RhoC knockdown spheres obtained from UM-SCC-1.