Maximum likelihood phylogenetic tree of <i>Pseudomonas gacA</i> genes.

<p><i>GacA</i> gene sequences of <i>E. fluviatilis</i>-derived <i>Pseudomonas</i> isolates (bold) and of most (>90%) isolates and clones present in relevant databases are shown. In brackets after the isolates' entries are their corresponding <i>gacA</i> operational taxonomic units (OTU) as inferred by rarefaction analysis at 97% similarity threshold (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088429#pone-0088429-g003" target="_blank">Fig. 3</a>). “Closest type” shows the 16S rRNA gene identity of the nearest type strain to relevant tree entries (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088429#pone.0088429.s001" target="_blank">Table S1</a> for a complete list and details). An asterisk indicates 100% 16S rRNA gene similarity between isolates and closest type strains. “Phylo group” refers to major, super-specific <i>Pseudomonas</i> phylogenetic lineages as determined by Mulet et al. (2010). Open and close circles on tree nodes correspond to bootstrap values ≥70% and 90%, respectively. The scale bar indicates 5% nucleotide substitution per site. The tree was rooted with the <i>gacA</i> gene homologue of <i>Burkholderia pseudomallei</i> strain K96243.