Neonatal CD103+ DCs differentially induce RSV-specific adaptive CD8+ T cell responses.

<p>A) CFSE-labeled TCR transgenic CD8+ T cells specific for K^dM2_82–90 or D^bM_187–195 were co-cultured in a 10∶1 ratio with CD103+ dendritic cells sorted from the lymph nodes of RSV infected adults or neonates one day after infection. The percent of cells that proliferated following three days in co-culture was calculated using the proliferation module in FlowJo 9.4.10. Negative control samples consisted of TCR Tg T cells co-cultured with splenocytes with no exogenous peptide, and positive control samples were co-cultured with splenocytes and 10⁻⁶M of specific peptide. B) Comparison of the ratio of proliferation (percent of K^dM2_82–90-specific cells that proliferated/percent of D^bM_187–195-specific cells that proliferated) induced by flow-sorted neonatal vs. adult CD103+DCs. Groups were compared using a student's t-test.