Binding of reduced and oxidized HMGB1 to histone H1 in free solution.

<p>H1 was mixed with reduced HMGB1 (lanes 1–4) or oxidized HMGB1 (lanes 5–8) at a molar ratio 1:1 and treated with dimethyl suberimidate for the times indicated. Cross-linking of (individual proteins) H1 (lanes 9–10), redHMGB1 (lanes 11–12) and oxHMGB1 (lanes 13–14) is shown. M, molecular mass marker; redHMGB1, reduced HMGB1 protein; oxHMGB1, oxidized HMGB1 protein. Gel was stained with silver.