The effects of insulin and LiCl treatments on FoxO1 subcellular localization.

<p>The subcellular localization of FoxO1 in C2C12-FoxO1-wt cells treated with/without insulin and LiCl for 2 and 4 days was detected with immunofluorescence microscopy as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088450#pone-0088450-g002" target="_blank">Fig. 2</a> and results are shown in (<b>A</b>) and (<b>B</b>) respectively. The relative percentages of myoblasts and multinucleated myotubes with either nuclear or cytoplasmic FoxO1 localization under various treatments were shown in (C) and (D). Arrow heads and arrows indicate MHC-positive myoblasts (mononucleate) and multinucleated myotubes, respectively. The original images were taken at 200X magnification. ud: undectable.