Detection of replication in HU-arrested cells.

<p>Cells synchronized in G1 phase were released in the presence of EdU or EdU plus HU. The cells were fixed after 75<b>A)</b> Fluorescence microscopy images of control cells at 0 minutes (top), EdU-labelled control cells at 75 minutes (middle) and EdU-labelled HU-treated cells at 75 minutes (bottom). <b>B)</b> Graph illustrating the relative fluorescent intensity in the samples indicated. The fluorescence intensity was measured using ImageJ 1.46 r in 50 cells at each timepoint. The background was set to the intensity measured at 0 min. The intensity measured in the 50 min sample was set to 100%.