Fecundity, fertility, and the development of embryos derived from irradiated adult females.

<p>(A–C) Fecundity and fertility of the wild type irradiated females. Two- to three-day-old adult females were irradiated with 0 or 40 Gy and the embryos were collected every 24 h. Day 1 corresponds to the egg collection between 0 and 24 h. The number of embryos per female (A) and the hatch rate of these embryos (B) were determined. Two independent experiments were performed using at least 5 cages for each treatment in the experiment, and the cage contained 3 pairs of females and males. The data are presented as the mean and standard deviation of the number of embryos per female and the hatch rate of those embryos was calculated from each cage. The grey X in (A) represents the percentage of egg production by irradiated females compared to untreated females. (C) The number of hatched larvae derived from one female was calculated and shown as above. (D–F) Development of the first instar larvae produced from irradiated females. (D–E) First instar larvae (n = 100) were obtained from the daily collection of eggs laid by the females treated with 0 or 40 Gy, and the number of pupae and eclosed adults was counted. The values represent the percentage of the total number of pupae formed (D) and the percentage of total number of adults generated from the pupae (E). The data are presented as the mean and standard deviation of three independent experiments. (F) The <i>Minute</i> phenotypes were observed in the adult females produced from females treated with 0 or 40 Gy. The percentage of adults with defective bristles is shown. The data are presented as the mean and standard deviation of a total of 42 females observed in three independent experiments.