IL-7Rα^449F/449F is not required for primary antibody responses.

<p>(A) Mice were immunized with NP-KLH or NP-LPS to assess T-dependent and T-independent responses, respectively. NP-specific IgG2b, IgG2c and IgM antibody responses to T-dependent (NP-KLH) and T-independent antigens (NP-LPS) were assessed by ELISA. (B) Using a NP-PE fluorescent label, numbers of NP-specific B220⁺ cells were assessed in the spleen of WT and IL-7Rα^449F/449F mice. Two separate sets of mice were analyzed. (C) WT, TSLPR^−/− and TSLPR^−/−IL-7Rα^449F/449F mice were infected with Influenza/A PR8 and strain-specific antibody responses in the serum and BAL were assessed by ELISA. n.d. = none detected. Three sets of mice were infected and analyzed. (D) WT, TSLPR^−/− and TSLPR^−/−IL-7Rα^449F/449F mice were orally infected with <i>H. polygyrus</i> and antibody responses to secreted antigen in serum (HES) were assessed by ELISA at 21 days. n.d. = none detected. Each symbol indicates an individual mouse and is representative of two separate experiments.