Mechanisms associated with the suppression by γδ DNT cells.

<p>(A) The suppression of HBV core peptide-induced production of TNF-α or IFN-γ in CD8⁺αβ T cells could be partly abrogated by inserting a membrane between the CD8⁺ αβ T cells and the γδ DNT cells in a transwell assay of cells from four patients with CHB. Lines link the data from the same patients. (B) γδ DNT cells were isolated and pre-incubated with either anti-NKG2A, anti-HLA-E, or mouse IgG1 isotype before being co-cultured with γδ T(−)PBMC. After 10 days of stimulation with peptides, production of TNF-α (black bars) and IFN-γ (blank bars) in CD8⁺αβ T cells was detected. Data from three patients are shown (core, HBV core peptides pool; gag, HIV gag peptide). DNT cells, double-negative T cells; IFN, interferon; PBMC, peripheral blood mononuclear cells; TNF, tumor necrosis factor.