GLA-AF augments priming of a protective immune response.

<p>Mice (5/group) received a prime immunization on d0 with rH5 VN (50 ng) either alone, with SE, or with GLA-AF (1 µg), and were then challenged 14 days later with 1000 LD50 A/H5N1/Vietnam/1203/04. (A) Mean percent change in body weight (+/− SEM) post-challenge (representative data from three experiments). (B) Kinetics of virus clearance. Each point is the average virus titer measured in mouse lung homogenate (n = 3), where 1 ml represents approximately 20% of lung volume. Statistical differences (p<0.05) between the adjuvanted and non-adjuvanted groups on day 3 and day 5 are indicated by the asterisks. (C) Day 14 sera were assayed for HI titers directed against the clade 1 vaccine HA or 2 heterosubtypic HA antigens. Each bar presents the geometric mean titer and statistical differences (p<0.001, Fisher’s test) between SE and GLA-AF are indicated. (D) Cumulative survival of mice immunized once with 50 ng of rH5 VN or rH5 Indo antigen and then challenged 14 days later with 1000 LD50 A/H5N1/Vietnam/1203/04.