Effect of diet on the expression of cholesterol biosynthesis genes.

<p><b>A</b> One-way hierarchical cluster map of cholesterol biosynthesis genes that changed significantly by at least one diet. mRNA expression of genes that were statistically different from those in mice fed the AIN-93 purified control diet have the percent indicated within the heatmap square (control = 100%). <b>B</b> RT-qPCR results validating the microarray result of Cyp7a1. Data statistically significant from control are denoted with an asterisk (*). <b>C</b> Western blot analysis of Cyp7a1 protein expression validating our mRNA findings. Top panel is a bar graph of the optical densities measured by ImageJ; bottom panel is representative western blots of Cyp7a1 and α-tubulin.