KLF4 expressions at both the transcriptional and the translational levels is drastically enhanced after treatment with the demethylating agent 5-Azacytidine.

<p>(A) Bisulfite sequencing of the KLF4 promoter in SiHa cells after treatment with different doses of 5-Aza. (B) KLF4 mRNA levels were quantified by PCR for three independent RNA samples from SiHa cells after treatment with different doses of 5-Aza, *, P<0.05. (C) KLF4 protein expression in SiHa cells was gradually enhanced in response to increasing doses of 5-Aza. (D) The relative expression of KLF4 protein in SiHa cells treated with different doses of 5-Aza. (E) KLF4 protein expression in SiHa cells was gradually enhanced during the time-course of treatment with 5 mM 5-Aza; it was reduced upon 5-Aza withdrawal following a 72-hour treatment. (F) Bisulfite sequencing of the KLF4 promoter in C33A cells after treatment with different doses of 5-Aza. (G and H) KLF4 expression was detected by PCR and western blot in C33A cells treated with different doses of 5-Aza in three independent repeats, *, P<0.05. (I) The relative expression of KLF4 protein in C33A cells treated with different doses of 5-Aza. (J) KLF4 protein expression was monitored during the time-course of treatment with 5 mM 5-Aza and during agent withdrawal following a 72-hour treatment. The relative levels of KLF4 protein normalized to β-actin are shown. Bars indicate SE. *, P<0.05.