Down-regulation of NNMT expression decreased the plate efficiency and attenuated the capacity of colony formation in soft ager

<p>(A, B) To test plate colony formation of Bcap-37 and MDA-MB-231 cells infected with NNMT shRNAs, cells were placed in wells with media and incubated for 14 days before counting the number of colonies (foci>50 µm). The plate efficiency of Bcap-37/NNMT shRNA 1# and Bcap-37/NNMT shRNA 2# cells were lower than that of Bcap-37/NC group. The similar result was found in MDA-MB-231 cell models (B). (C, D) Colony formation of Bcap-37 and MDA-MB-231 infected with NNMT shRNAs was carried out by placing cells in media containing soft ager for 14 days. The numbers of Bcap-37/NNMT shRNA 1# and Bcap-37/NNMT shRNA foci >100 µm were less than that of Bcap-37/NC group (**<i>P</i><0.01). The similar result was found in MDA-MB-231 cell models (D). Values are expressed as means ± SD of four independent experiments.