Collagen quantification.

<p>(A) Collagen deposition was detected by picrosirius red staining in tissue sections 21 days post MI and CSp transplantation. Representative tissue images under bright field on the left and under polarized light microscope on the right. (B) Quantification of the collagen in the total LV area and C. separately in the infarct, border and remote zone. (D,E) Results of RT-PCR for mRNA expression of coll1a1 and coll3a1 respectively. (F) Hydroxyproline assay performed for collagen content production in the infarct, border, and in the remote zone. Significant differences between the two groups were observed in the infarct and in the border areas. (G) ICTP quantification in the serum collected 7 days post MI and treatment with increased levels detected in the treated group. Abbreviations. IZ (infarct zone), BZ (border zone), RZ (remote zone). Data are mean±SEM. ¶ p<0.05 control vs. MI+CSp, * p<0.05 vs. sham.