Isolation and characterization of MSCs derived from human decidua.

<p>(A): Schematic description of the way to isolate MSCs. (B): Morphology of MSCs isolate after 4 day, 6 day and 8 day (20X). (C) Cell growth curve of MSCs. UCMSCs of passage 2 were plated in 24-well plates in DMEM/F12 supplemented with 10% FBS at a density of 5×10³ cells/well. The cells were detached with 0.25% trypsin-EDTA every 2 days and cell counting until 14 day. Bars show the mean. n = 3. (D) Osteogenic differentiation was assayed by the alizarin red staining. No mineralized matrix formation was found in UCMSCs maintained in regular growth medium. Osteogenic differentiation was evidenced by the formation of mineralized matrix in UCMSCs after osteogeneic induction. (10X). Scale bars, 100 µm. (E) Adipogenic differentiation was detected by oil red O staining. No lipid vacuoles were found in in UCMSCs maintained in the regular medium. Adipogenic differentiation was evidenced by the formation of lipid vacuoles by oil-red O staining in UCMSCs after adipogenic induction. (10X) Scale bars, 100 µm. All data are representative of three independent experiments.