Binding kinetic analysis of wild type and mutant versions of Sli15 to immobilized microtubules by biolayer interferometry.

<p>A comparison of the binding abilities between recombinant GST-Sli15-His₆ and its mutants to 5 µM taxol-stabilized, biotinylated polymerized tubulin, immobilized to streptavidin-coated biosensors is shown. (A) Binding results for five different concentrations of wild-type and mutant Sli15 (see inset) with equivalent concentrations of recombinant GST protein used as a control. Data shown are representative of three separate experiments. (B) Binding of wild type Sli15 and Ipl1 alone or in combination to taxol-stabilized, biotinylated polymerized tubulin with or without Sli15 phosphorylation by Ipl1 (+ATP); see inset for key. Data shown are representative of two separate experiments.