Coreceptor usage and entry efficiency of recombinant pseudotyped HIV.

<p>Coreceptor usage of the recombinant luciferase-reporter HIV was determined using NP2/CD4 cells expressing either CCR5 or CXCR4. Entry efficiencies of recombinant pseudotyped HIVs were determined using NP2/CD4 cells expressing both CXCR4 and CCR5. The cells were infected with the same amount of luciferase-reporter pseudotyped virus (10 ng p24Ag) with the indicated mutations. Luciferase activities were measured at 48 h post-infection. Data are the geometric means ± SD of triplicate experiments.