Western blot analysis of Bcl-2 family proteins and survival pathway related proteins.

<p><b>Panel A.</b> Western blot analysis of Bax, Bcl-2, Phospho-Akt, PI3k proteins in liver tissue homogenates. β actin was used as an internal control. Cont: normal mice, PNP: mice treated with only PNP, ASA: mice intoxicated with aspirin, ASA+PNP: mice treated with PNP after aspirin intoxication. ASA+VitC: mice treated with vitamin C after aspirin intoxication. “a” indicates the significant difference between the normal control and ASA intoxicated groups, and “b” indicates the significant difference between ASA intoxicated (toxin) and PNP post-treated groups. Each column represents mean ± SD, n = 6; (P^a<0.05, P^b<0.05). <b>Panel B.</b> Western blot analysis of Bax, Bcl-2, Phospho-Akt, PI3k proteins in spleen tissue homogenates. β actin was used as an internal control. Cont: normal mice, PNP: mice treated with only PNP, ASA: mice intoxicated with aspirin, ASA+PNP: mice treated with PNP after aspirin intoxication. “a” indicates the significant difference between the normal control and ASA intoxicated groups, and “b” indicates the significant difference between ASA intoxicated (toxin) and PNP post-treated groups. Each column represents mean ± SD, n = 6; (P^a<0.05, P^b<0.05).