Induction of NCAM and ΔPD-PAX6 by M2 macrophages are overlapping phenotypes associated with enhanced migration.

<p>(A) Flow cytometric analysis of G3LC, SU86 and PANC-1 cell lines co-cultured with either M1 (M1 macs) or M2 (M2 macs) macrophages, immuno-stained for NCAM and the pan-leukocyte marker CD45. The contour plots shows cells gated as CD45^neg (i.e. epithelial cells only), and the percentage of NCAM⁺ cells are indicated in the right lower quadrant. (B) Migration of G3LC cells sorted from co-cultures with M2 macrophages as CD45^negNCAM^Neg and CD45^negNCAM^Pos subsets, in response to 20% FCS. Bars represent the mean ± SD of duplicate samples in each experiment. *<i>p</i><0.001. (C) Western blotting analysis of PAX6 isoforms expressed in cytoplasmic cell lysates from sorted NCAM^Neg and NCAM^Pos G3LC cells derived from the M2 macrophages co-cultures shown in panel A.