ENT1 null mice have reduced trabecular bone volume fraction, thickness and number and increased trabecular separation in the femur by micro computerized tomography (microCT).

<p>Representative cross section through the trabecular bone of the distal femoral metaphysis of the femur of (<b>A</b>) wild-type and (<b>B</b>) ENT1 null mice. Scale bar = 1 mm. <b>C</b> Reduced trabecular bone volume fraction (BV/TV), which is equal to the percentage of the tissue space that is occupied by bone within the lumbar vertebral body, of ENT1 null mice compared to wild-type mice. <b>D</b> Trabecular thickness (Tb.Th) or the average thickness of the trabecular struts was reduced in ENT1 null mice compared to wild-type littermates. <b>E</b> Trabecular number (Tb.N) or a measure of the number of trabeculae within a given space was decreased in ENT1 null mice relative to wild-type mice. <b>F</b> Trabecular separation (Tb.S) or the average spacing between adjacent trabeculae was reduced in ENT1 null compared to wild-type mice. <i>n</i> = 3 per genotype. *<i>P</i><0.05 by Student’s two-tailed <i>t</i> test. All data are presented as mean ± SEM.