Disjunction persists at 14 days post-fracture in GIT1 KO mice.

<p>Femur fractures were induced in 10-week-old WT and GIT1 KO mice. Fractured femora were harvested for analysis at 7, 14, and 21 days post-injury. Radiographs obtained at the 14 day time point consistently revealed radiolucency in GIT1 KO calluses (B, red arrow) compared with calluses from WT mice (A, yellow arrow). This was supported by microCT analysis, which revealed lack of bridging mineral in GIT1 KOs (D, red arrows) compared to a connected shell of mineral in WT controls (C). Further quantification of callus geometry via microCT indicated that there were no differences in mineralized callus volume between WT and GIT1 KO mice (E). Bars represent mean callus volume (mm³) +/− SEM (N = 3, *p<0.05).