Native LPETG substrate incorporation does not induce the srtA KO hyper-spreading phenotype in the WT S. aureus strain.

<p>SH1000 WT and its isogenic <i>srtA</i> KO <i>S. aureus</i> strains were cultured in TSB in the presence of either medium, native or scrambled LPETG substrates during 24 hrs. The final substrate concentration was 2 mM, 1 mM or 0.5 mM. After the incubation individual cultures were spotted on the TSA soft agar and incubated during 20 hrs. The spreading zones were examined and pictures were taken after the incubation. These are representative images of three independent experiments.