Effects of NSC and 2-Brp on HSP25 and HSP70 protein levels under heat shock conditions.

<p>Cells were treated as described for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0089136#pone-0089136-g005" target="_blank"><b>Figure 5</b></a> and were collected after an overnight recovery at 37°C. (<b>A</b>) Western blotting was performed for equal amounts of total protein samples. Membranes were probed with anti-HSP25, anti-HSP70 and anti-Gapdh antibodies. (<b>B</b>) The band intensities of protein expression levels were quantitated and normalized to Gapdh. In calculations of fold changes, the protein levels of 41.5°C samples were taken as 100. Data are means ± SD, n = 3.