Effect of BPA and Nar on ERα activities.

<p>(Panel A) Western blot analysis of ERα S118 phosphorylation in MCF-7 cells treated with vehicle (C), E2, Nar or BPA for 2 hrs and relative densitometry. The same filter was re-probed with anti-ERα antibody. Loading control was performed by evaluating vinculin expression levels. * indicates significant differences with respect to the control sample. RT-qPCR analysis of presenelin 2 (pS2/TIFF) (panel B), progesterone receptor (PR) (panel C) and cathepsin D (CatD) (panel D) mRNA expression in MCF-7 cells treated with E2, Nar or BPA for 24 hrs. * indicates significant differences with respect to the control sample; ° indicates significant differences with respect to the E2-treated samples. (Panel E) HeLa cells were transfected with ERα wild type or with ERα S118A mutant together with a plasmid containing 3×ERE-TATA-luciferase construct. Cells were treated with vehicle E2, Nar or BPA for 24 hrs and the promoter activity was evaluated as described in material and method section. * indicates significant differences with respect to the control sample; ° indicates significant differences with respect to the E2-treated samples; # indicates significant differences with respect to the correspondent wild type samples.